Chromogenic Substrates

CHROMOGENIC SUBSTRATES For In Vitro Research Studies.

If you do not find the substrate you are looking for, please contact us at: info@aniara.com

The BIOPHEN® line of chromogenic substrates is a new line of high quality chromogenic substrates, which allow testing enzymes involved in coagulation and fibrinolysis. The BIOPHEN line of substrates are of the highest quality and stability. The Hyphen Line of Substrates carry all the traditional substrates for Thrombin, Factor Xa, Factor IIa, Kallikrein, Activated Protein C, Plasmin and Plasminogen-SK, and many more. To find out the chemical composition of the substrate please click on more information on that substrate.

These substrates offer prolonged stability following reconstitution.
Aniara can provide substrates in bulk format and for industrial use.
Methods have been developed for Factor Xa and Factor IIa assays.
We can validate your methods with our reagents – at our lab in France.

Enzymes are proteins that catalyze most of the chemical reactions that take place in the body. They make it possible for chemical reactions to occur at neutral pH and body temperature. The chemical compound upon which the enzyme exerts its catalytic activity is called a substrate. Proteolytic enzymes act on their natural substrates, proteins and peptides by hydrolyzing one or more peptide bond(s). This process is usually highly specific in the sense that only peptide bonds adjacent to certain amino acids are cleaved.

Chromogenic substrates are peptides that react with proteolytic enzymes under the formation of color. They are made synthetically and are designed to possess a selectivity similar to that of the natural substrate for the enzyme. Attached to the peptide part of the chromogenic substrate is a chemical group which when released after the enzyme cleavage gives rise to color. The color change can be followed spectrophotometrically and is proportional to the proteolytic activity.

Chromogenic substrate technology was developed in the early 1970s, and has since then become a tool of substantial importance in basic research. The majority of chromogenic substrate applications are found in various clinical fields. In particular they have been used to generate fundamental knowledge of the mechanisms regulating elastase, mast cell tryptase, and many of the factors regulating blood coagulation and fibrinolysis. Furthermore, products based on chromogenic substrate technology have brought a new generation of diagnostics into the clinical laboratory. These substrates offer a prolonged stability following reconstitution. We can also offer these substrates in 1g and 5g format.

Evaluation of Hyphen BioMed Chromogenic Substrates vs Chromogenix Substrates

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Product	Specification	Application
BIOPHEN CS-01(38) - Thrombin	Peptide Sequence: H-D-Phe-Pip-Arg-pNa, 2HCl Molecular Weight: 552.6 Da Chemical Structure: C27H36N8O5, 2HCl	Chromogenic Substrate for Thrombin
BIOPHEN CS-05(88) - tPA & broad spectrum	Peptide Sequence: H-D-Ile--Pro-Arg-pNa-2HCl Molecular Weight: 504.6.	Synthetic Generic Chromogenic Substrate
BIOPHEN CS-11(32) - Factor Xa	Peptide Sequence: Suc-Ile-Gly (γ Pip)Gly-Arg-pNa, HCl Molecular Weight: 760.9 Da Chemical Structure: C34H52N10O10, HCl	Chromogenic Substrate for Factor Xa
BIOPHEN CS-11(65) Anti-Xa	Peptide Sequence: D-Arg-Gly-Arg-pNA, 2HCl Molecular Weight: Da Chemical Formula: C28H39N11O7, 2HCl	Chromogenic Substrate for Factor Xa
BIOPHEN CS-11(22) - Factor Xa	Form 1(50%) Peptide Sequence: Bz-Ile-Glu(g-OH)-Gly-Arg-pNA. Molecular Weight: 711.8 Da Chemical Sequence: C33H45N9O9, HCl Form 2 (50%): Peptide Sequence: Bz-Ile-Glu(g-OH)-Gly-Arg-pNA Molecular Weight: 697.7 Da Chemical Sequence: C32H43N9O9, HCl	Chromogenic Substrate for Factor Xa
BIOPHEN CS-21(66) - Activated Protein C	Peptide Sequence: Glu-Pro-Arg-pNa, HCl Molecular Weight: 502.5 Da Chemical Structure: C22H30N6O6, HCl	Chromogenic Substrate for Activated Protein C
BIOPHEN CS-31(02) - Kallicrein	Peptide Sequence: D-Pro-Phe-Arg-pNa, 2HCl Molecular Weight: 538.6 Da Chemical Structure: C26H34N8O5, 2HC	Chromogenic Substrate for Kallicrein
BIOPHEN CS-41(03) - Plasmin and Plasminogen-SK	Peptide Sequence: PyroGlu-Phe-Lys-pNa, HCl Molecular Weight: 524.6 Da Chemical Structure: C`26`H32N6O6, HCl	Chromogenic Substrate for Plasmin and Plasminogen-SK
BIOPHEN FIXa CS 51(09) - Factor IXa	Peptide Sequence: CH3SO2-(D-)CHG-Gly-Arg-pNA Molecular Weight: 628.7 Da	Chromogenic Substrate for Factor IXa
BIOPHEN CS-61(44) Urokinase	Peptide Sequence: pyro-Glu-Gly-Arg-pNa.HCl Molecular weight: 462.5 Chemical Structure: C19H26N8O6, HCl.	Synthetic Generic Chromogenic Substrate
FLUOROGENIC FLUOPHEN FIXa	Peptide Sequence: H-(D)-Leu-PHG-Arg-AMC.2AcOH Molecular Weight: 696.8 Da	Synthetic Fluorognic Substrate for Testing Factor IXa

**Purchase Products:**
BIOPHEN CS-01 (81) - CHROMOZYME TH	Product Details
BIOPHEN CS-01(38) - Thrombin	Product Details
BIOPHEN CS-05(88) - tPA and broad spectrum	Product Details
BIOPHEN CS-11(22) - Factor Xa	Product Details
BIOPHEN CS-11(32) - Factor Xa	Product Details
BIOPHEN CS-11(65) Anti-Xa	Product Details
BIOPHEN CS-21(66) - Activated Protein C	Product Details
BIOPHEN CS-31(02) - Kallicrein	Product Details
BIOPHEN CS-41(03) - Plasmin and Plasminogen-SK	Product Details
BIOPHEN FIXa CS 51(09) - Factor IXa	Product Details
FLUOROGENIC FLUOPHEN FIXa	Product Details
BIOPHEN CS-61(44) Urokinase	Product Details